SEQUENCE_ID=case01_full_design
SEQUENCE_TEMPLATE=GTAGCTAGCTCCAGTGGCAATTGCACCAGGTTTGCAAGCTAGCATCGGATCCGAATTCGGTACCAAGCTTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATTGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCT
PRIMER_TASK=generic
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_OPT_SIZE=20
PRIMER_MIN_SIZE=18
PRIMER_MAX_SIZE=22
PRIMER_OPT_TM=60.0
PRIMER_MIN_TM=57.0
PRIMER_MAX_TM=63.0
PRIMER_MIN_GC=30.0
PRIMER_MAX_GC=70.0
PRIMER_PRODUCT_SIZE_RANGE=100-250
PRIMER_NUM_RETURN=5
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=1
PRIMER_THERMODYNAMIC_TEMPLATE_ALIGNMENT=0
PRIMER_THERMODYNAMIC_PARAMETERS_PATH=/var/folders/zv/8z3736f56736py6cytnhldkm0000gn/T/tmp.PtpVsa3Opx/primer3_config/
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 977, GC content failed 13, low tm 283, high tm 163, long poly-x seq 17, ok 501
PRIMER_RIGHT_EXPLAIN=considered 977, GC content failed 138, low tm 198, high tm 326, long poly-x seq 17, ok 298
PRIMER_INTERNAL_EXPLAIN=considered 2692, GC content failed 13, low tm 1030, high tm 643, high hairpin stability 1, long poly-x seq 22, ok 983
PRIMER_PAIR_EXPLAIN=considered 6, unacceptable product size 1, ok 5
PRIMER_LEFT_NUM_RETURNED=5
PRIMER_RIGHT_NUM_RETURNED=5
PRIMER_INTERNAL_NUM_RETURNED=5
PRIMER_PAIR_NUM_RETURNED=5
PRIMER_PAIR_0_PENALTY=0.074185
PRIMER_LEFT_0_PENALTY=0.038021
PRIMER_RIGHT_0_PENALTY=0.036164
PRIMER_INTERNAL_0_PENALTY=0.248895
PRIMER_LEFT_0_SEQUENCE=TAGCTCCAGTGGCAATTGCA
PRIMER_RIGHT_0_SEQUENCE=CCCATTCGCCAATCCGGATA
PRIMER_INTERNAL_0_SEQUENCE=CTGCTGCCACCGCTGAGCAA
PRIMER_LEFT_0=5,20
PRIMER_RIGHT_0=216,20
PRIMER_INTERNAL_0=110,20
PRIMER_LEFT_0_TM=59.962
PRIMER_RIGHT_0_TM=59.964
PRIMER_INTERNAL_0_TM=60.249
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_INTERNAL_0_GC_PERCENT=65.000
PRIMER_INTERNAL_0_SELF_ANY_TH=1.65
PRIMER_LEFT_0_SELF_ANY_TH=30.32
PRIMER_RIGHT_0_SELF_ANY_TH=19.48
PRIMER_INTERNAL_0_SELF_END_TH=0.00
PRIMER_LEFT_0_SELF_END_TH=14.71
PRIMER_RIGHT_0_SELF_END_TH=0.00
PRIMER_LEFT_0_HAIRPIN_TH=44.36
PRIMER_RIGHT_0_HAIRPIN_TH=36.56
PRIMER_INTERNAL_0_HAIRPIN_TH=35.78
PRIMER_LEFT_0_END_STABILITY=4.0800
PRIMER_RIGHT_0_END_STABILITY=2.5900
PRIMER_PAIR_0_COMPL_ANY_TH=0.00
PRIMER_PAIR_0_COMPL_END_TH=4.08
PRIMER_PAIR_0_PRODUCT_SIZE=212
PRIMER_PAIR_0_PRODUCT_TM=87.1
PRIMER_PAIR_1_PENALTY=0.135599
PRIMER_LEFT_1_PENALTY=0.099436
PRIMER_RIGHT_1_PENALTY=0.036164
PRIMER_INTERNAL_1_PENALTY=0.248895
PRIMER_LEFT_1_SEQUENCE=GGTTTGCAAGCTAGCATCGG
PRIMER_RIGHT_1_SEQUENCE=CCCATTCGCCAATCCGGATA
PRIMER_INTERNAL_1_SEQUENCE=CTGCTGCCACCGCTGAGCAA
PRIMER_LEFT_1=28,20
PRIMER_RIGHT_1=216,20
PRIMER_INTERNAL_1=110,20
PRIMER_LEFT_1_TM=59.901
PRIMER_RIGHT_1_TM=59.964
PRIMER_INTERNAL_1_TM=60.249
PRIMER_LEFT_1_GC_PERCENT=55.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_INTERNAL_1_GC_PERCENT=65.000
PRIMER_INTERNAL_1_SELF_ANY_TH=1.65
PRIMER_LEFT_1_SELF_ANY_TH=18.83
PRIMER_RIGHT_1_SELF_ANY_TH=19.48
PRIMER_INTERNAL_1_SELF_END_TH=0.00
PRIMER_LEFT_1_SELF_END_TH=4.02
PRIMER_RIGHT_1_SELF_END_TH=0.00
PRIMER_LEFT_1_HAIRPIN_TH=45.19
PRIMER_RIGHT_1_HAIRPIN_TH=36.56
PRIMER_INTERNAL_1_HAIRPIN_TH=35.78
PRIMER_LEFT_1_END_STABILITY=4.1800
PRIMER_RIGHT_1_END_STABILITY=2.5900
PRIMER_PAIR_1_COMPL_ANY_TH=0.00
PRIMER_PAIR_1_COMPL_END_TH=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=189
PRIMER_PAIR_1_PRODUCT_TM=86.8
PRIMER_PAIR_2_PENALTY=0.142499
PRIMER_LEFT_2_PENALTY=0.106335
PRIMER_RIGHT_2_PENALTY=0.036164
PRIMER_INTERNAL_2_PENALTY=0.248895
PRIMER_LEFT_2_SEQUENCE=TGGCAATTGCACCAGGTTTG
PRIMER_RIGHT_2_SEQUENCE=CCCATTCGCCAATCCGGATA
PRIMER_INTERNAL_2_SEQUENCE=CTGCTGCCACCGCTGAGCAA
PRIMER_LEFT_2=14,20
PRIMER_RIGHT_2=216,20
PRIMER_INTERNAL_2=110,20
PRIMER_LEFT_2_TM=59.894
PRIMER_RIGHT_2_TM=59.964
PRIMER_INTERNAL_2_TM=60.249
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_INTERNAL_2_GC_PERCENT=65.000
PRIMER_INTERNAL_2_SELF_ANY_TH=1.65
PRIMER_LEFT_2_SELF_ANY_TH=30.32
PRIMER_RIGHT_2_SELF_ANY_TH=19.48
PRIMER_INTERNAL_2_SELF_END_TH=0.00
PRIMER_LEFT_2_SELF_END_TH=0.00
PRIMER_RIGHT_2_SELF_END_TH=0.00
PRIMER_LEFT_2_HAIRPIN_TH=44.36
PRIMER_RIGHT_2_HAIRPIN_TH=36.56
PRIMER_INTERNAL_2_HAIRPIN_TH=35.78
PRIMER_LEFT_2_END_STABILITY=2.9300
PRIMER_RIGHT_2_END_STABILITY=2.5900
PRIMER_PAIR_2_COMPL_ANY_TH=0.00
PRIMER_PAIR_2_COMPL_END_TH=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=203
PRIMER_PAIR_2_PRODUCT_TM=86.9
PRIMER_PAIR_3_PENALTY=0.142685
PRIMER_LEFT_3_PENALTY=0.106521
PRIMER_RIGHT_3_PENALTY=0.036164
PRIMER_INTERNAL_3_PENALTY=0.248895
PRIMER_LEFT_3_SEQUENCE=GTGGCAATTGCACCAGGTTT
PRIMER_RIGHT_3_SEQUENCE=CCCATTCGCCAATCCGGATA
PRIMER_INTERNAL_3_SEQUENCE=CTGCTGCCACCGCTGAGCAA
PRIMER_LEFT_3=13,20
PRIMER_RIGHT_3=216,20
PRIMER_INTERNAL_3=110,20
PRIMER_LEFT_3_TM=59.893
PRIMER_RIGHT_3_TM=59.964
PRIMER_INTERNAL_3_TM=60.249
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_INTERNAL_3_GC_PERCENT=65.000
PRIMER_INTERNAL_3_SELF_ANY_TH=1.65
PRIMER_LEFT_3_SELF_ANY_TH=30.32
PRIMER_RIGHT_3_SELF_ANY_TH=19.48
PRIMER_INTERNAL_3_SELF_END_TH=0.00
PRIMER_LEFT_3_SELF_END_TH=0.00
PRIMER_RIGHT_3_SELF_END_TH=0.00
PRIMER_LEFT_3_HAIRPIN_TH=44.36
PRIMER_RIGHT_3_HAIRPIN_TH=36.56
PRIMER_INTERNAL_3_HAIRPIN_TH=35.78
PRIMER_LEFT_3_END_STABILITY=3.2700
PRIMER_RIGHT_3_END_STABILITY=2.5900
PRIMER_PAIR_3_COMPL_ANY_TH=0.00
PRIMER_PAIR_3_COMPL_END_TH=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=204
PRIMER_PAIR_3_PRODUCT_TM=87.0
PRIMER_PAIR_4_PENALTY=0.143818
PRIMER_LEFT_4_PENALTY=0.107654
PRIMER_RIGHT_4_PENALTY=0.036164
PRIMER_INTERNAL_4_PENALTY=0.248895
PRIMER_LEFT_4_SEQUENCE=GTACCAAGCTTGATCCGGCT
PRIMER_RIGHT_4_SEQUENCE=CCCATTCGCCAATCCGGATA
PRIMER_INTERNAL_4_SEQUENCE=CTGCTGCCACCGCTGAGCAA
PRIMER_LEFT_4=59,20
PRIMER_RIGHT_4=216,20
PRIMER_INTERNAL_4=110,20
PRIMER_LEFT_4_TM=60.108
PRIMER_RIGHT_4_TM=59.964
PRIMER_INTERNAL_4_TM=60.249
PRIMER_LEFT_4_GC_PERCENT=55.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_INTERNAL_4_GC_PERCENT=65.000
PRIMER_INTERNAL_4_SELF_ANY_TH=1.65
PRIMER_LEFT_4_SELF_ANY_TH=28.05
PRIMER_RIGHT_4_SELF_ANY_TH=19.48
PRIMER_INTERNAL_4_SELF_END_TH=0.00
PRIMER_LEFT_4_SELF_END_TH=4.03
PRIMER_RIGHT_4_SELF_END_TH=0.00
PRIMER_LEFT_4_HAIRPIN_TH=40.85
PRIMER_RIGHT_4_HAIRPIN_TH=36.56
PRIMER_INTERNAL_4_HAIRPIN_TH=35.78
PRIMER_LEFT_4_END_STABILITY=5.5200
PRIMER_RIGHT_4_END_STABILITY=2.5900
PRIMER_PAIR_4_COMPL_ANY_TH=6.82
PRIMER_PAIR_4_COMPL_END_TH=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=158
PRIMER_PAIR_4_PRODUCT_TM=86.2
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