SEQUENCE_ID=case02_classic_pcr_no_thermo
SEQUENCE_TEMPLATE=GTAGCTAGCTCCAGTGGCAATTGCACCAGGTTTGCAAGCTAGCATCGGATCCGAATTCGGTACCAAGCTTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGATTGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCT
PRIMER_TASK=generic
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=0
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_OPT_SIZE=20
PRIMER_MIN_SIZE=18
PRIMER_MAX_SIZE=23
PRIMER_PRODUCT_SIZE_RANGE=120-200
PRIMER_NUM_RETURN=3
PRIMER_THERMODYNAMIC_OLIGO_ALIGNMENT=0
PRIMER_EXPLAIN_FLAG=1
PRIMER_LEFT_EXPLAIN=considered 1047, low tm 255, high tm 249, high end compl 1, long poly-x seq 18, ok 524
PRIMER_RIGHT_EXPLAIN=considered 1047, GC content failed 13, low tm 186, high tm 563, high end compl 1, long poly-x seq 18, ok 266
PRIMER_PAIR_EXPLAIN=considered 4, unacceptable product size 1, ok 3
PRIMER_LEFT_NUM_RETURNED=3
PRIMER_RIGHT_NUM_RETURNED=3
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=3
PRIMER_PAIR_0_PENALTY=0.221044
PRIMER_LEFT_0_PENALTY=0.099436
PRIMER_RIGHT_0_PENALTY=0.121608
PRIMER_LEFT_0_SEQUENCE=GGTTTGCAAGCTAGCATCGG
PRIMER_RIGHT_0_SEQUENCE=TTAGAGGCCCCAAGGGGTTA
PRIMER_LEFT_0=28,20
PRIMER_RIGHT_0=158,20
PRIMER_LEFT_0_TM=59.901
PRIMER_RIGHT_0_TM=59.878
PRIMER_LEFT_0_GC_PERCENT=55.000
PRIMER_RIGHT_0_GC_PERCENT=55.000
PRIMER_LEFT_0_SELF_ANY=8.00
PRIMER_RIGHT_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=2.00
PRIMER_LEFT_0_END_STABILITY=4.1800
PRIMER_RIGHT_0_END_STABILITY=2.8500
PRIMER_PAIR_0_COMPL_ANY=3.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=131
PRIMER_PAIR_0_PRODUCT_TM=85.8
PRIMER_PAIR_1_PENALTY=0.227944
PRIMER_LEFT_1_PENALTY=0.106335
PRIMER_RIGHT_1_PENALTY=0.121608
PRIMER_LEFT_1_SEQUENCE=TGGCAATTGCACCAGGTTTG
PRIMER_RIGHT_1_SEQUENCE=TTAGAGGCCCCAAGGGGTTA
PRIMER_LEFT_1=14,20
PRIMER_RIGHT_1=158,20
PRIMER_LEFT_1_TM=59.894
PRIMER_RIGHT_1_TM=59.878
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=8.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=2.9300
PRIMER_RIGHT_1_END_STABILITY=2.8500
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=145
PRIMER_PAIR_1_PRODUCT_TM=86.1
PRIMER_PAIR_2_PENALTY=0.228130
PRIMER_LEFT_2_PENALTY=0.106521
PRIMER_RIGHT_2_PENALTY=0.121608
PRIMER_LEFT_2_SEQUENCE=GTGGCAATTGCACCAGGTTT
PRIMER_RIGHT_2_SEQUENCE=TTAGAGGCCCCAAGGGGTTA
PRIMER_LEFT_2=13,20
PRIMER_RIGHT_2=158,20
PRIMER_LEFT_2_TM=59.893
PRIMER_RIGHT_2_TM=59.878
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=1.00
PRIMER_RIGHT_2_SELF_END=2.00
PRIMER_LEFT_2_END_STABILITY=3.2700
PRIMER_RIGHT_2_END_STABILITY=2.8500
PRIMER_PAIR_2_COMPL_ANY=3.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PAIR_2_PRODUCT_SIZE=146
PRIMER_PAIR_2_PRODUCT_TM=86.3
=
